ABSTRACT
With the COVID-19 pandemic, life satisfaction among college students has become a key issue at universities and in society. The current study explores the effects of belief in a just world and resilience on the relationship between relative deprivation and life satisfaction. A total of 787 college students from universities in China completed online questionnaires. Results showed that relative deprivation was negatively correlated with life satisfaction. Belief in a just world and resilience separately mediated the relationship between relative deprivation and life satisfaction. Moreover, a serial mediating effect of belief in a just world and resilience was observed between relative deprivation and life satisfaction. These findings suggest that relative deprivation may impair individuals' beliefs in a just world. Moreover, less belief in a just world may lower resilience and consequently decrease life satisfaction. This study enriches the research field of relative deprivation theory in the context of the COVID-19 pandemic, and provides a new interpretation and intervention perspective for improving college students' life satisfaction.
ABSTRACT
The outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) caused a global health emergency, and its gene mutation and evolution further posed uncertainty of epidemic risk. Herein, we reported a light-up CRISPR-Cas13 transcription amplification method, which enables the detection of SARS-CoV-2 and its mutated variants. Sequence specificity was ensured by both the ligation process and Cas13a/crRNA recognition, allowing us to identify viral RNA mutation. Light-up RNA aptamer allows sensitive output of amplification signals via target-activated ribonuclease activity of CRISPR-Cas13a. The RNA virus assay has been designed to detect coronavirus, SARS-CoV-2, Middle East respiratory syndrome (MERS), and SARS, as well as the influenza viruses such as, H1N1, H7N9, and H9N2. It was accommodated to sense as low as 82 copies of SARS-CoV-2. Particularly, it allowed us to strictly discriminate key mutation of the SARS-CoV-2 variant, D614G, which may induce higher epidemic and pathogenetic risk. The proposed RNA virus assays are promising for point-of-care monitoring of SARS-CoV-2 and its risking variants.
Subject(s)
COVID-19 Nucleic Acid Testing/methods , COVID-19/virology , CRISPR-Associated Proteins/genetics , CRISPR-Cas Systems/genetics , Clustered Regularly Interspaced Short Palindromic Repeats/genetics , SARS-CoV-2/isolation & purification , Humans , Molecular Diagnostic Techniques , Mutation , Nucleic Acid Amplification Techniques , RNA, Viral/genetics , SARS-CoV-2/geneticsABSTRACT
During January-February 2020, coronavirus disease (COVID-19) and tuberculosis were diagnosed for 3 patients in Wuhan, China. All 3 patients had COVID-19 pneumonia. One severely ill patient died after acute respiratory distress syndrome developed. Clinicians and public health officials should be aware of underlying chronic infections such as tuberculosis in COVID-19 patients.